Generator
Part:BBa_K131010:Design
Designed by: Kevin McLeod Group: iGEM08_Calgary_Wetware (2008-07-28)
AHL Inducible Colicin E2 with GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3736
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1004
Illegal BsaI.rc site found at 1731
Design Notes
GFP (I13500) had to be placed before the colicin E2 operon because of the intrinsic terminator present in the operon. Otherwise little to no GFP would be produced.
Source
F2620 and I13500 were preexisting parts from the registry. See K131000 for information on the colicin genes.