Generator

Part:BBa_K131010:Design

Designed by: Kevin McLeod   Group: iGEM08_Calgary_Wetware   (2008-07-28)


AHL Inducible Colicin E2 with GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3736
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 1731


Design Notes

GFP (I13500) had to be placed before the colicin E2 operon because of the intrinsic terminator present in the operon. Otherwise little to no GFP would be produced.


Source

F2620 and I13500 were preexisting parts from the registry. See K131000 for information on the colicin genes.

References